THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT intelligence 453 : BIOLOGY FOR ENGINEERS REPORT No.1 BACTERIA CLASSIFICATION BY yard STAINING Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â bearing: Â Â Â Â Â Â Â Â To test a sample of testing ground watchful bacterium and categorise it according to Christians constant of gravitation collateral and gram negative classes and also by viewing it on a lower floor a high powered microscope and oil immersions; classify its spurt and note any finical characteristics. Introduction: Â Â Â Â Â Â Â Â Bacteria was categorised into deuce groups in 1884 by the Danish Bacteriologist Christian, gram positive and gram negative by a staining technique where the shootice to avoid de-coloration of crystal over-embellished solution by alcoholic beverage woul d render the home of gram positive, and gram negative if the bacteria is de- colour ined. This could be far-famed by the final colour of the bacteria: a imperial colour where g positive and a pink colour of the safranin added pending the de-colouring process. Materials: 1. Bacteria Sample 2. Microscope Slide 3. universal gravitational constant Staining Kit and Wash Bottles a. Crystal Violet upshot b. iodin Solution c. 95% ethyl radical Alcohol d. Safranin e. Dis cashboxed Water 4. Bibulous Blotting Paper 5. Microscope 6. Oil Procedure: A.
re adiness : 1. Bacteria is civilised o! n agar jelly in an incubator at 25°C for 24 hours. 2. take hold a microscope sea-coast and with a toothpick, taint a thinly coat of the bacteria sample onto the slide 3. Cover the smear with a drop Crystal Violet and pass outdoor(a) standing for 20 seconds 4. Wash off the stain with distilled water supply; drain and blot off the unneeded with bibulous paper. 5. drill Grams Iodine on the smear and leave to stand for 1 minute. 6. Drain the excess iodine and gain 95% Ethyl alcohol for 20 second while or till the alcohol runs clearly from... If you want to aim a exuberant essay, order it on our website:
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