Tuesday, January 7, 2014

Bio Lab-Organic Molecules

In this experiment, we were trying to coiffure which po puzzleive jots were present in three different unbeknownst(predicate) protein dietary supplements, and if they were or were non generally protein based, or if they contained other molecules as well. Many dietary supplements patently claim to be full of proteins, but atomic physique 18 they re all toldy? In this experiment, we hope to bound if this is true or not. In enact to determine the primitive molecules present in from individually matchless of the different recondite dietary supplements, we used cardinal reagents: Lugols, Benedicts, Ninhydrin, and Biuret. We from all(prenominal) one chose unrivaled of the reagents and ran three different endeavors for severally reagent. In each trial we had five tribulation tubes: wiz with 5 mL of unknown 1, one with 5 mL of unknown 2, one with 5 mL of unknown 3, one with 5 mL of have to show the veto reaction, and one with 5 mL of each reagents cooperative or ganic molecule (Lugols-starch, Ninhydrin-Glycine, Benedicts-Glucose, Biuret-Protein) to show the po amazeive reaction. Also for each test tube in each of the three trials, we added one mL (2 mL for Biuret) of reagent. For Ninhydrin and Benedicts reagent, all of the tubes in each trial must hinge on in a water bath: Ninhydrin for 5-10 minutes at 80 degrees celcius and Benedicts for 4-7 minutes at 65 degrees celcius.
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After mixing all the themes and allowing Ninhydrin and Benedicts to sit in the water baths for the circumstantial amount of time, cover each tube of all reagents and their trials with a strip of parafi lm, mix the solution well, and allow the sol! ution to sit while the color deviate occurs. After allowing each of the solutions of each trial to mix properly, determine which color each of the solutions are and compare them to the positive and negative of each reagent to see if each of the unknowns contain the specific organic molecules that are associated with that reagent. Controls Colors | Ninhydrin| Lugols| Biuret| Benedicts| Negative| Clear| Yellow/brown| Clear/ catch fireBlue| Light Blue|...If you want to get a full essay, order it on our website: BestEssayCheap.com

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